Cilia: Structure and Motility

Cryo-Electron Microscope Tomography to Study Axonemal

Organization

Daniela Nicastro

2. Electron Microscopic Imaging and Analysis of Isolated

Dynein Particles

Anthony J. Roberts and Stan A. Burgess

3. Immunogold Labeling of Flagellar Components In Situ

Stefan Geimer

4. Scanning Electron Microscopy to Examine Cells and Organs

Jovenal T. SanAgustin, John A. Follit, Gregory Hendricks, and Gregory J. Pazour

5. X-ray Fiber Diffraction Studies on Flagellar Axonemes

Kazuhiro Oiwa, Shinji Kamimura, and Hiroyuki Iwamoto

6. Markers for Neuronal Cilia

Jacqueline S. Domire and Kirk Mykytyn

7. Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells

Heymut Omran and Niki T. Loges

8. Immunoprecipitation to Examine Protein Complexes

Gregory J. Pazour

9. Tandem Affinity Purification of Ciliopathy-Associated Protein

Complexes

Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner,

Marius Ueffing, and Ronald Roepman

10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products

Miho Sakato

11. Analysis of the Ciliary/Flagellar Beating of Chlamydomonas

Kenneth W. Foster

12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii

Ritsu Kamiya

13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain

Karl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman

14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow

Yasushi Okada and Nobutaka Hirokawa

15. Modification of Mouse Nodal Flow by Applying Artificial Flow

Shigenori Nonaka

16. Measuring Cilium-Induced Ca2þ Increases in Cultured Renal Epithelia

Helle A. Praetorius