Cilia, Part B

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.

Hardbound, 514 Pages

Published: April 2013

Imprint: Academic Press

ISBN: 978-0-12-397944-5

Contents

    1. Methods for studying ciliary polarity in Xenopus
      Brian Mitchell
    2. Analysis of signaling pathways in mammalian spermatozoa
      Marco Conti
    3. Analysis of tubulin modification in zebrafish cilia
      Iain Drummond
    4. Analysis of microtubule end-binding proteins in cilia
      Lotte B. Pedersen
    5. Small molecule screens using Chlamydomonas flagella
      Prachee Avasthi
    6. Biochemical and Physiological analysis of axonemal dyneins
      Stephen King
    7. Analysis of ciliary assembly and function in Planaria
      Panteleimon Rompolas
    8. Reconstitution of flagellar sliding
      Jonathon Howard
    9. Comparative genomic analysis of ciliary genes
      Susan Dutcher
    10. Analysis of ciliogenesis in Tetrahymena
      Jacek Gaertig
    11. Measuring the regulation of dynein activity during flagellar motility
      Chikako Shingyoji
    12. Centrosome isolation and proteomics
      Jens S. Andersen
    13. Probing ciliogenesis using micropatterned substrates
      Manuel Thery
    14. 3D structural analysis of flagella/cilia by electron cryo-tomography
      Takashi Ishikawa
    15. Visualizing IFT in mammalian cilia
      Jagesh Shah
    16. Ciliogenesis in multiciliated epithelial cells in culture
      Eszter Katalin Vladar
    17. Methods for analysis of PKD-containing urinary exosomes
      Christopher J. Ward
    18. Analysis of Interactions between IFT Proteins
      Douglas Cole
    19. Electron microscopy of Intraflagellar Transport
      Pietro Lupetti
    20. Using the SNAP tag to measure ciliary trafficking
      Gregory J. Pazour

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