Cilia, Part B book cover

Cilia, Part B

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 498 Pages

Published: April 2013

Imprint: Academic Press

ISBN: 978-0-12-397944-5

Contents

    1. Methods for studying ciliary polarity in Xenopus
    2. Brian Mitchell

    3. Analysis of signaling pathways in mammalian spermatozoa
    4. Marco Conti

    5. Analysis of tubulin modification in zebrafish cilia
    6. Iain Drummond

    7. Analysis of microtubule end-binding proteins in cilia
    8. Lotte B. Pedersen

    9. Small molecule screens using Chlamydomonas flagella
    10. Prachee Avasthi

    11. Biochemical and Physiological analysis of axonemal dyneins
    12. Stephen King

    13. Analysis of ciliary assembly and function in Planaria
    14. Panteleimon Rompolas

    15. Reconstitution of flagellar sliding
    16. Jonathon Howard

    17. Comparative genomic analysis of ciliary genes
    18. Susan Dutcher

    19. Analysis of ciliogenesis in Tetrahymena
    20. Jacek Gaertig

    21. Measuring the regulation of dynein activity during flagellar motility
    22. Chikako Shingyoji

    23. Centrosome isolation and proteomics
    24. Jens S. Andersen

    25. Probing ciliogenesis using micropatterned substrates
    26. Manuel Thery

    27. 3D structural analysis of flagella/cilia by electron cryo-tomography
    28. Takashi Ishikawa

    29. Visualizing IFT in mammalian cilia
    30. Jagesh Shah

    31. Ciliogenesis in multiciliated epithelial cells in culture
    32. Eszter Katalin Vladar

    33. Methods for analysis of PKD-containing urinary exosomes
    34. Christopher J. Ward

    35. Analysis of Interactions between IFT Proteins
    36. Douglas Cole

    37. Electron microscopy of Intraflagellar Transport
    38. Pietro Lupetti

    39. Using the SNAP tag to measure ciliary trafficking

    Gregory J. Pazour

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