Cilia, Part B

Edited by

  • Wallace F. Marshall, Department of Biochemistry & Biophysics, University of California San Fransisco at Mission Bay, USA

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.
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Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Book information

  • Published: April 2013
  • ISBN: 978-0-12-397944-5

Table of Contents

  1. Methods for studying ciliary polarity in Xenopus
  2. Brian Mitchell

  3. Analysis of signaling pathways in mammalian spermatozoa
  4. Marco Conti

  5. Analysis of tubulin modification in zebrafish cilia
  6. Iain Drummond

  7. Analysis of microtubule end-binding proteins in cilia
  8. Lotte B. Pedersen

  9. Small molecule screens using Chlamydomonas flagella
  10. Prachee Avasthi

  11. Biochemical and Physiological analysis of axonemal dyneins
  12. Stephen King

  13. Analysis of ciliary assembly and function in Planaria
  14. Panteleimon Rompolas

  15. Reconstitution of flagellar sliding
  16. Jonathon Howard

  17. Comparative genomic analysis of ciliary genes
  18. Susan Dutcher

  19. Analysis of ciliogenesis in Tetrahymena
  20. Jacek Gaertig

  21. Measuring the regulation of dynein activity during flagellar motility
  22. Chikako Shingyoji

  23. Centrosome isolation and proteomics
  24. Jens S. Andersen

  25. Probing ciliogenesis using micropatterned substrates
  26. Manuel Thery

  27. 3D structural analysis of flagella/cilia by electron cryo-tomography
  28. Takashi Ishikawa

  29. Visualizing IFT in mammalian cilia
  30. Jagesh Shah

  31. Ciliogenesis in multiciliated epithelial cells in culture
  32. Eszter Katalin Vladar

  33. Methods for analysis of PKD-containing urinary exosomes
  34. Christopher J. Ward

  35. Analysis of Interactions between IFT Proteins
  36. Douglas Cole

  37. Electron microscopy of Intraflagellar Transport
  38. Pietro Lupetti

  39. Using the SNAP tag to measure ciliary trafficking

Gregory J. Pazour