Cilia, Part B book cover

Cilia, Part B

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 498 Pages

Published: April 2013

Imprint: Academic Press

ISBN: 978-0-12-397944-5

Contents

    1. Methods for studying ciliary polarity in Xenopus
      Brian Mitchell

    2. Analysis of signaling pathways in mammalian spermatozoa
      Marco Conti

    3. Analysis of tubulin modification in zebrafish cilia
      Iain Drummond

    4. Analysis of microtubule end-binding proteins in cilia
      Lotte B. Pedersen

    5. Small molecule screens using Chlamydomonas flagella
      Prachee Avasthi

    6. Biochemical and Physiological analysis of axonemal dyneins
      Stephen King

    7. Analysis of ciliary assembly and function in Planaria
      Panteleimon Rompolas

    8. Reconstitution of flagellar sliding
      Jonathon Howard

    9. Comparative genomic analysis of ciliary genes
      Susan Dutcher

    10. Analysis of ciliogenesis in Tetrahymena
      Jacek Gaertig

    11. Measuring the regulation of dynein activity during flagellar motility
      Chikako Shingyoji

    12. Centrosome isolation and proteomics
      Jens S. Andersen

    13. Probing ciliogenesis using micropatterned substrates
      Manuel Thery

    14. 3D structural analysis of flagella/cilia by electron cryo-tomography
      Takashi Ishikawa

    15. Visualizing IFT in mammalian cilia
      Jagesh Shah

    16. Ciliogenesis in multiciliated epithelial cells in culture
      Eszter Katalin Vladar

    17. Methods for analysis of PKD-containing urinary exosomes
      Christopher J. Ward

    18. Analysis of Interactions between IFT Proteins
      Douglas Cole

    19. Electron microscopy of Intraflagellar Transport
      Pietro Lupetti

    20. Using the SNAP tag to measure ciliary trafficking
      Gregory J. Pazour

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