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Cilia, Part A

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.

Hardbound, 436 Pages

Published: April 2013

Imprint: Academic Press

ISBN: 978-0-12-397945-2

Contents

          1. Electron microscopy of IFT in cilia and flagella
            Stefan Geimer

          2. Biochemical analysis of ciliary transport

            3. Peter Jackson

          3. Visualization of IFT in Chlamydomonas flagella

            4. Karl F. Lechtreck

          4. Visualization of kidney cilia-mediated calcium signaling

            5. Jing Zhou

          5. Methods for analyzing nodal cilia and flow

            6. Shigenori Nonaka

          6. Methods for building synthetic cilia-like motor-microtubule arrays

            7. Zvonimir Dogic

          7. Isolation of IFT particle proteins from Chlamydomonas

            8. Hongmin Qin

          8. Radial Spoke isolation and assays

            9. Pinfen Yang

          9. Biomechanical measurements of kinocilium

            10. Wally Grant

          10. Methods for analysis of Calcium/calmodulin signaling in flagella

            11. Elizabeth Smith

          11. Ubiquitination of Flagellar Proteins

            12. Kaiyao Huang Sr.

          12. Visualizing IFT in C. elegans cilia

            13. Jonathan M. Scholey

          13. Proteomic analysis of mammalian primary cilia

            14. Hiroaki Ishikawa

          14. TSC and TOR signaling in cilia

            15. Zhaoxia Sun

          15. Bioinformatic approaches to cilia

            16. Peter Swoboda

          16. Methods for testing cilia function in cell migration

            17. Søren Tvorup Christensen

          17. Signaling and waveform in Drosophila sperm flagella

            18. Xiangyi Lu

          18. Analysis of import into primary cilia

            19. Kristen Verhey

          19. Patch-Clamp Recording from Mouse and Human Spermatozoa

            20. Yuriy Kirichok

          20. Optical trap measurements of flagellar membrane motility
            William Guilford

     

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