Cilia, Part A

Edited by

  • Wallace F. Marshall, Department of Biochemistry & Biophysics, University of California San Fransisco at Mission Bay, USA

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.
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Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Book information

  • Published: April 2013
  • ISBN: 978-0-12-397945-2

Table of Contents

        1. Electron microscopy of IFT in cilia and flagella
          Stefan Geimer
        2. Biochemical analysis of ciliary transport
        3. Peter Jackson

        4. Visualization of IFT in Chlamydomonas flagella
        5. Karl F. Lechtreck

        6. Visualization of kidney cilia-mediated calcium signaling
        7. Jing Zhou

        8. Methods for analyzing nodal cilia and flow
        9. Shigenori Nonaka

        10. Methods for building synthetic cilia-like motor-microtubule arrays
        11. Zvonimir Dogic

        12. Isolation of IFT particle proteins from Chlamydomonas
        13. Hongmin Qin

        14. Radial Spoke isolation and assays
        15. Pinfen Yang

        16. Biomechanical measurements of kinocilium
        17. Wally Grant

        18. Methods for analysis of Calcium/calmodulin signaling in flagella
        19. Elizabeth Smith

        20. Ubiquitination of Flagellar Proteins
        21. Kaiyao Huang Sr.

        22. Visualizing IFT in C. elegans cilia
        23. Jonathan M. Scholey

        24. Proteomic analysis of mammalian primary cilia
        25. Hiroaki Ishikawa

        26. TSC and TOR signaling in cilia
        27. Zhaoxia Sun

        28. Bioinformatic approaches to cilia
        29. Peter Swoboda

        30. Methods for testing cilia function in cell migration
        31. Søren Tvorup Christensen

        32. Signaling and waveform in Drosophila sperm flagella
        33. Xiangyi Lu

        34. Analysis of import into primary cilia
        35. Kristen Verhey

        36. Patch-Clamp Recording from Mouse and Human Spermatozoa
        37. Yuriy Kirichok

        38. Optical trap measurements of flagellar membrane motility
          William Guilford