Cilia, Part A book cover

Cilia, Part A

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 472 Pages

Published: April 2013

Imprint: Academic Press

ISBN: 978-0-12-397945-2

Contents

          1. Electron microscopy of IFT in cilia and flagella
            Stefan Geimer
          2. Biochemical analysis of ciliary transport
          3. Peter Jackson

          4. Visualization of IFT in Chlamydomonas flagella
          5. Karl F. Lechtreck

          6. Visualization of kidney cilia-mediated calcium signaling
          7. Jing Zhou

          8. Methods for analyzing nodal cilia and flow
          9. Shigenori Nonaka

          10. Methods for building synthetic cilia-like motor-microtubule arrays
          11. Zvonimir Dogic

          12. Isolation of IFT particle proteins from Chlamydomonas
          13. Hongmin Qin

          14. Radial Spoke isolation and assays
          15. Pinfen Yang

          16. Biomechanical measurements of kinocilium
          17. Wally Grant

          18. Methods for analysis of Calcium/calmodulin signaling in flagella
          19. Elizabeth Smith

          20. Ubiquitination of Flagellar Proteins
          21. Kaiyao Huang Sr.

          22. Visualizing IFT in C. elegans cilia
          23. Jonathan M. Scholey

          24. Proteomic analysis of mammalian primary cilia
          25. Hiroaki Ishikawa

          26. TSC and TOR signaling in cilia
          27. Zhaoxia Sun

          28. Bioinformatic approaches to cilia
          29. Peter Swoboda

          30. Methods for testing cilia function in cell migration
          31. Søren Tvorup Christensen

          32. Signaling and waveform in Drosophila sperm flagella
          33. Xiangyi Lu

          34. Analysis of import into primary cilia
          35. Kristen Verhey

          36. Patch-Clamp Recording from Mouse and Human Spermatozoa
          37. Yuriy Kirichok

          38. Optical trap measurements of flagellar membrane motility
            William Guilford

     

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