Cilia, Part A book cover

Cilia, Part A

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 472 Pages

Published: April 2013

Imprint: Academic Press

ISBN: 978-0-12-397945-2

Contents

    1. Electron microscopy of IFT in cilia and flagella
      Stefan Geimer

    2. Biochemical analysis of ciliary transport
      Peter Jackson

    3. Visualization of IFT in Chlamydomonas flagella
      Karl F. Lechtreck

    4. Visualization of kidney cilia-mediated calcium signaling
      Jing Zhou

    5. Methods for analyzing nodal cilia and flow
      Shigenori Nonaka

    6. Methods for building synthetic cilia-like motor-microtubule arrays
      Zvonimir Dogic

    7. Isolation of IFT particle proteins from Chlamydomonas
      Hongmin Qin

    8. Radial Spoke isolation and assays
      Pinfen Yang

    9. Biomechanical measurements of kinocilium
      Wally Grant

    10. Methods for analysis of Calcium/calmodulin signaling in flagella
      Elizabeth Smith

    11. Ubiquitination of Flagellar Proteins
      Kaiyao Huang Sr.

    12. Visualizing IFT in C. elegans cilia
      Jonathan M. Scholey

    13. Proteomic analysis of mammalian primary cilia
      Hiroaki Ishikawa

    14. TSC and TOR signaling in cilia
      Zhaoxia Sun

    15. Bioinformatic approaches to cilia
      Peter Swoboda

    16. Methods for testing cilia function in cell migration
      Søren Tvorup Christensen

    17. Signaling and waveform in Drosophila sperm flagella
      Xiangyi Lu

    18. Analysis of import into primary cilia
      Kristen Verhey

    19. Patch-Clamp Recording from Mouse and Human Spermatozoa
      Yuriy Kirichok

    20. Optical trap measurements of flagellar membrane motility
      William Guilford

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