Cilia: Motors and Regulation

Edited by
  • Stephen King, University of Texas, MD Anderson Cancer Center,Michale E. Keeling Center for Comparative Medicine and Research, Bastrop, Texas, U.S.A.
  • Gregory Pazour

Included in series
Methods in Cell Biology

Hardbound, 400 Pages

Published: November 2009

Imprint: Academic Press

ISBN: 978-0-12-374974-1

Contents

  • MCB, 92

    1. Bioinformatic Approaches to Dynein Heavy Chain Classification

    Toshiki Yagi

    2. Identification and Characterization of Dynein Genes in Tetrahymena

    David E. Wilkes, Nicole Bennardo, Clarence W.C. Chan, Yu-Loung Chang,

    Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime, Julianna Erickson,

    Jonathan Hetzel, Erin E. Heyer, Mark J. Hubenschmidt, Ekaterina Kniazeva,

    Hallie Kuhn, Michelle Lum, Andrea Sand, Alicia Schep, Oksana Sergeeva,

    Natt Supab, Caroline R. Townsend, Liesl Van Ryswyk, Hadley E. Watson,

    Alice E. Wiedeman, Vidyalakshmi Rajagopalan, and David J. Asai

    3. Purification of Axonemal Dyneins and Dynein-Associated Components

    from Chlamydomonas

    Stephen M. King

    4. Purification of Dyneins from Sperm Flagella

    Kazuo Inaba and Katsutoshi Mizuno

    5. Protein Engineering Approaches to Study the Dynein Mechanism

    using a Dictyostelium Expression System

    Takahide Kon, Tomohiro Shima, and Kazuo Sutoh

    6. Biophysical Measurements on Axonemal Dyneins

    Hiroaki Kojima, Shiori Toba, Hitoshi Sakakibara, and Kazuhiro Oiwa

    7. Protein Electroporation into Chlamydomonas for Mutant Rescue

    Masahito Hayashi and Ritsu Kamiya

    8. Analysis of the Role of Nucleotides in Axonemal Dynein Function

    Chikako Shingyoji

    9. The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas

    Flagella by Axonemal Kinases and Phosphatases

    Candice A. Elam, Winfield S. Sale, and Maureen Wirschell

    10. Analysis of Redox-Sensitive Dynein Components

    Ken-ichi Wakabayashi

    11. Calcium Regulation of Ciliary Motility: Analysis of Axonemal

    Calcium-Binding Proteins

    Christen DiPetrillo and Elizabeth Smith

    12. Isolation and Analysis of Radial Spoke Proteins

    Pooja Kelekar, Mei Wei, and Pinfen Yang

    13. Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii

    David R. Mitchell and Brandon Smith

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