- Richard Horuk
- John Abelson
- Melvin Simon
Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human diseases and infections. This volume and its companion Volume 288 on Chemokine Receptors provide comprehensive experimental protocols used in this field of research.
Biochemists, molecular biologists, immunologists, cell biologists, pharmacologists, and biomedical researchers.
Methods in Enzymology
Hardbound, 421 Pages
Published: September 1997
Imprint: Academic Press
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- C-X-C Chemokines:R. Horuk, D. Reilly, and D. Yansura, Expression, Purification, and Characterization of Escherichia coli Derived Recombinant Human Melanoma Growth Stimulating Activity.A. Wuyts, P. Proost,G. Froyen, A. Haelens, A. Billiau, G. Opdenakker, and J. Van Damme, Purification and Identification of Human and Mouse Granulocyte Chemotactic Protein-2 Isoforms.P.E. Dawson, Synthesis of Chemokines by Native Chemical Ligation.W.J. Fairbrother and H.B. Lowman, Molecular Approaches to Structure-Function Analysis of Interleukin-8.J. Hesselgesser and R. Horuk, Alanine Scan Mutagenesis of Chemokines.D. Baly, U. Gibson, D. Allison, and L. DeForge, Biological Assays for C-X-C Chemokines.K. Rajarathnam, C.M. Kay, I. Clark-Lewis, and B.D. Sykes, Characterization of Quaternary Structure of Interleukin-8 and Functional Implications.C-C and C Chemokines:J. Van Damme, S. Struyf, A. Wuyts, G. Opdenakker, P. Proost, P. Allavena, S. Sozzani, and A. Mantovani, Isolation of Human Monocyte Chemotactic Proteins and Study of their Producer and Responder Cells by Immunotests and Bioassays.A.-M. Buckle, S. Craig, and L.G. Czaplewski, Assays for Macrophage Inflammatory Proteins.P.J. Nelson, J.M. Pattison, and A.M. Krensky, Gene Expression of RANTES.A.M. Krensky and P.J. Nelson, Expression of Chemokine RANTES and Production of Monoclonal Antibodies.R.C. Newton and K. Vaddi, Biological Responses to C-C Chemokines.D.N. Cook, Gene Targeting Strategies to Study Chemokine Function in Vivo.J.A. Hedrick and A. Zlotnik, Lymphotactin: A New Class of Chemokine.J.M. Schrider, Isolation and Purification of Neutrophil Activating Peptide 4: A Chemokine Missing Two Cysteines.Other Methods:I. Clark-Lewis, L. Vo, P. Owen, and J. Anderson, Chemical Synthesis Purification and Folding of C-X-C and C-C Chemokines.J.B. Smith and H.R. Herschmann, Identification of Inflammatory Mediators by Screening for Glucocorticoid-Attenuated Response Genes.D.D. Taub, M.L. Key, D.L. Longo, and W.J. Murphy, Chemokine-Induced Human Lymphocyte Infiltration and Engraftment in huPBL-SCID Mice.D.A. Barnes and H.D. Perez, HighThroughput Screening for Identification of RANTES Chemokine Expression Inhibitors.S.A. Lira, M.E. Fuentes, R.M. Strieter, and S.K. Durham, Transgenic Methods to Study Chemokine Function in Lung and Central Nervous System.R.M. Ransohoff, M Tani, A.R. Glabinski, A. Chernosky, K. Krivacic, J.W. Peterson, H.-F. Chien, and B.D. Trapp, Chemokines and Chemokine Receptors in Model Neurological Pathologies: Molecular and Immunocytochemical Approaches.R.E. Offord, H.F. Gaertner, T.N.C. Wells, andA.E.I. Proudfoot, Synthesis and Evaluation of Fluorescent Chemokines Labeled at the Amino Terminal.G.S.V. Kuschert, R.E. Hubbard, C.A. Power, T.N.C. Wells, and A.J. Hoogewerf, Solid-Phase Binding Assay to Study Interaction of Chemokines with Glycosaminoglycans.M. Conklyn and H.J. Showell, Biological Activity of C-X-C and C-C Chemokines in Leukocyte Subpopulations in Human Whole Blood.Author Index.Subject Index.