Cellulases book cover

Cellulases

Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."

Audience
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 552 Pages

Published: May 2012

Imprint: Academic Press

ISBN: 978-0-12-415931-0

Contents

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    1. Measurement of endo-1,4-b-glucanase
    2. Barry V. McCleary, Vincent McKie and Anna Draga

    3. Biomass Conversion determined via Fluorescent Cellulose Decay assay
    4. Bente Wischmann, Marianne Toft, Marco Malten and KC McFarland

    5. The analysis of saccharification in biomass using an automated high-throughput method
    6. Caragh Whitehead, Leonardo D. Gomez, Simon J. McQueen-Mason

    7. Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
    8. Ondrej Kosik, Jennifer Bromley, Marta Busse-Wicher, Zhinong Zhang & Paul Dupree

    9. Measuring processivity
    10. Svein J. Horn, Morten Sørlie, Kjell M. Vårum, Priit Väljamäe and Vincent G.H. Eijsink

    11. Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases
    12. Jens M. Eklöf, Marcus C. Ruda and Harry Brumer

    13. Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
    14. Maria J. Peña, Sami T. Tuomivaara, Breeanna R. Urbanowicz, Malcolm A. O’Neill and William S. York

    15. The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
    16. Shirley M. Roberts and Gideon J. Davies

    17. Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
    18. Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Merja Penttilä, Toshio Ando and Masahiro Samejima

    19. Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
    20. Mirjam Czjzek, Henri-Pierre Fierobe and Véronique Receveur-Bréchot

    21. Quantitative approaches to the analysis of carbohydrate-binding module function
    22. D. Wade Abbott‡ and Alisdair B. Boraston

    23. In Situ Detection Of Cellulose With Carbohydrate-Binding Modules
    24. J. Paul Knox

    25. Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides
    26. Oren Yaniv. Felix Frolow. Maly Levy-Assraf, Raphael Lamed and Edward A. Bayer

    27. Approaches for improving thermostability characteristics in cellulases
    28. Michael Anbar and Edward A. Bayer

    29. Thermophilic glycosynthases for oligosaccharides synthesis
    30. Beatrice Cobucci-Ponzano, Giuseppe Perugino, Andrea Strazzulli, Mosè Rossi and Marco Moracci

    31. Engineering cellulase activity into Clostridium acetobutylicum
    32. Henri-Pierre Fierobe, Florence Mingardon and Angélique Chanal

    33. Transformation of Clostridium thermocellum by electroporation
    34. Daniel G. Olson and Lee R. Lynd

    35. Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
    36. Jeffrey G. Gardner and David H. Keating

    37. Methods for the isolation of cellulose-degrading microorganisms
    38. James E. McDonald, David J. Rooks and Alan J. McCarthy

    39. Metagenomic approaches to the discovery of cellulases
    40. David J. Rooks, James E. McDonald and Alan J. McCarthy

    41. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes
    42. Joana Brás, Ana Luisa Carvalho, Aldino Viegas, Shabir Najmudin, Victor D. Alves, José A.M. Prates, Luís M.A. Ferreira, Maria J. Romão, Harry J. Gilbert and Carlos M.G.A. Fontes

    43. Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions
    44. Michal Slutzki, Yoav Barak, Dan Reshef, Ora Schueler-Furman, Raphael Lamed and Edward A. Bayer

    45. Designer cellulosomes for enhanced hydrolysis of cellulosic substrates
    46. Yael Vazana, Sarah Moraïs, Yoav Barak, Raphael Lamed and Edward A. Bayer

    47. High-throughput screening of cohesin mutant libraries on cellulose microarrays

    Michal Slutzki, Vered Ruimy, Ely Morag, Yoav Barak, Rachel Haimovitz, Raphael Lamed and Edward A. Bayer

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