Cellulases book cover


Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 552 Pages

Published: May 2012

Imprint: Academic Press

ISBN: 978-0-12-415931-0


    1. Measurement of endo-1,4-b-glucanase
      Barry V. McCleary, Vincent McKie and Anna Draga

    2. Biomass Conversion determined via Fluorescent Cellulose Decay assay
      Bente Wischmann, Marianne Toft, Marco Malten and KC McFarland

    3. The analysis of saccharification in biomass using an automated high-throughput method
      Caragh Whitehead, Leonardo D. Gomez, Simon J. McQueen-Mason

    4. Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
      Ondrej Kosik, Jennifer Bromley, Marta Busse-Wicher, Zhinong Zhang & Paul Dupree

    5. Measuring processivity
      Svein J. Horn, Morten Sørlie, Kjell M. Vårum, Priit Väljamäe and Vincent G.H. Eijsink

    6. Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases
      Jens M. Eklöf, Marcus C. Ruda and Harry Brumer

    7. Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
      Maria J. Peña, Sami T. Tuomivaara, Breeanna R. Urbanowicz, Malcolm A. O’Neill and William S. York

    8. The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
      Shirley M. Roberts and Gideon J. Davies

    9. Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
      Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Merja Penttilä, Toshio Ando and Masahiro Samejima

    10. Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
      Mirjam Czjzek, Henri-Pierre Fierobe and Véronique Receveur-Bréchot

    11. Quantitative approaches to the analysis of carbohydrate-binding module function
      D. Wade Abbott‡ and Alisdair B. Boraston

    12. In Situ Detection Of Cellulose With Carbohydrate-Binding Modules
      J. Paul Knox

    13. Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides
      Oren Yaniv. Felix Frolow. Maly Levy-Assraf, Raphael Lamed and Edward A. Bayer

    14. Approaches for improving thermostability characteristics in cellulases
      Michael Anbar and Edward A. Bayer

    15. Thermophilic glycosynthases for oligosaccharides synthesis
      Beatrice Cobucci-Ponzano, Giuseppe Perugino, Andrea Strazzulli, Mosè Rossi and Marco Moracci

    16. Engineering cellulase activity into Clostridium acetobutylicum
      Henri-Pierre Fierobe, Florence Mingardon and Angélique Chanal

    17. Transformation of Clostridium thermocellum by electroporation
      Daniel G. Olson and Lee R. Lynd

    18. Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
      Jeffrey G. Gardner and David H. Keating

    19. Methods for the isolation of cellulose-degrading microorganisms
      James E. McDonald, David J. Rooks and Alan J. McCarthy

    20. Metagenomic approaches to the discovery of cellulases
      David J. Rooks, James E. McDonald and Alan J. McCarthy

    21. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes
      Joana Brás, Ana Luisa Carvalho, Aldino Viegas, Shabir Najmudin, Victor D. Alves, José A.M. Prates, Luís M.A. Ferreira, Maria J. Romão, Harry J. Gilbert and Carlos M.G.A. Fontes

    22. Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions
      Michal Slutzki, Yoav Barak, Dan Reshef, Ora Schueler-Furman, Raphael Lamed and Edward A. Bayer

    23. Designer cellulosomes for enhanced hydrolysis of cellulosic substrates
      Yael Vazana, Sarah Moraïs, Yoav Barak, Raphael Lamed and Edward A. Bayer

    24. High-throughput screening of cohesin mutant libraries on cellulose microarrays
      Michal Slutzki, Vered Ruimy, Ely Morag, Yoav Barak, Rachel Haimovitz, Raphael Lamed and Edward A. Bayer


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