Cellulases

Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."
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Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

 

Book information

  • Published: May 2012
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-415931-0


Table of Contents

 

  1. Measurement of endo-1,4-b-glucanase
  2. Barry V. McCleary, Vincent McKie and Anna Draga

  3. Biomass Conversion determined via Fluorescent Cellulose Decay assay
  4. Bente Wischmann, Marianne Toft, Marco Malten and KC McFarland

  5. The analysis of saccharification in biomass using an automated high-throughput method
  6. Caragh Whitehead, Leonardo D. Gomez, Simon J. McQueen-Mason

  7. Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
  8. Ondrej Kosik, Jennifer Bromley, Marta Busse-Wicher, Zhinong Zhang & Paul Dupree

  9. Measuring processivity
  10. Svein J. Horn, Morten Sørlie, Kjell M. Vårum, Priit Väljamäe and Vincent G.H. Eijsink

  11. Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases
  12. Jens M. Eklöf, Marcus C. Ruda and Harry Brumer

  13. Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
  14. Maria J. Peña, Sami T. Tuomivaara, Breeanna R. Urbanowicz, Malcolm A. O’Neill and William S. York

  15. The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
  16. Shirley M. Roberts and Gideon J. Davies

  17. Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
  18. Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Merja Penttilä, Toshio Ando and Masahiro Samejima

  19. Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
  20. Mirjam Czjzek, Henri-Pierre Fierobe and Véronique Receveur-Bréchot

  21. Quantitative approaches to the analysis of carbohydrate-binding module function
  22. D. Wade Abbott‡ and Alisdair B. Boraston

  23. In Situ Detection Of Cellulose With Carbohydrate-Binding Modules
  24. J. Paul Knox

  25. Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides
  26. Oren Yaniv. Felix Frolow. Maly Levy-Assraf, Raphael Lamed and Edward A. Bayer

  27. Approaches for improving thermostability characteristics in cellulases
  28. Michael Anbar and Edward A. Bayer

  29. Thermophilic glycosynthases for oligosaccharides synthesis
  30. Beatrice Cobucci-Ponzano, Giuseppe Perugino, Andrea Strazzulli, Mosè Rossi and Marco Moracci

  31. Engineering cellulase activity into Clostridium acetobutylicum
  32. Henri-Pierre Fierobe, Florence Mingardon and Angélique Chanal

  33. Transformation of Clostridium thermocellum by electroporation
  34. Daniel G. Olson and Lee R. Lynd

  35. Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
  36. Jeffrey G. Gardner and David H. Keating

  37. Methods for the isolation of cellulose-degrading microorganisms
  38. James E. McDonald, David J. Rooks and Alan J. McCarthy

  39. Metagenomic approaches to the discovery of cellulases
  40. David J. Rooks, James E. McDonald and Alan J. McCarthy

  41. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes
  42. Joana Brás, Ana Luisa Carvalho, Aldino Viegas, Shabir Najmudin, Victor D. Alves, José A.M. Prates, Luís M.A. Ferreira, Maria J. Romão, Harry J. Gilbert and Carlos M.G.A. Fontes

  43. Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions
  44. Michal Slutzki, Yoav Barak, Dan Reshef, Ora Schueler-Furman, Raphael Lamed and Edward A. Bayer

  45. Designer cellulosomes for enhanced hydrolysis of cellulosic substrates
  46. Yael Vazana, Sarah Moraïs, Yoav Barak, Raphael Lamed and Edward A. Bayer

  47. High-throughput screening of cohesin mutant libraries on cellulose microarrays

Michal Slutzki, Vered Ruimy, Ely Morag, Yoav Barak, Rachel Haimovitz, Raphael Lamed and Edward A. Bayer