Cell Biological Applications of Confocal Microscopy

Edited by

  • Brian Matsumoto, Neuroscience Research Institute, University of California, Santa Barbara, U.S.A.

Confocal Microscopy contains integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Techniques are described completely so that methods are made accessible to users. This is a must-have reference for all CM users.
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Cell biologists, molecular biologists, optical scientists; biophysicists; neuroscientists, developmental biologists, and researchers and companies involved in the various types of microscopy and imaging discussed in the work.


Book information

  • Published: December 2002
  • ISBN: 978-0-12-580445-5


Praise for the First Edition: "...the desired aim of providing the confocal user with a technical basis for preparing superior microscpic samples is achieved." —TRENDS IN NEUROSCIENCES Praise for the Series: "The series is invaluable for workers at all levels of cell biology." —NATURE

Table of Contents

Introduction to Confocal Microscopy. Direct-View High-Speed Confocal Scanner - the CSU-10; Introduction to Two-Photon Microscopes.Confocal Microscopy: Important Considerations for Accurate Imaging. Multicolor-Laser Scanning Confocal Immunofluorescence Microscopy: Practical Application and Limitations. Practical Aspects of Objective Lens Selection for Confocal and Multi-Photon Digital Imaging Techniques. Resolution of Subcellular Detail in Thick Tissue Sections: Immunohistochemical Preparation and Fluorescence Confocal Microscopy. Confocal Fluorescence Microscopy for Tetrahymena. Confocal Imaging for Drosophila Embryos. Confocal Fluorescence Microscopy of the Cytoskeleton of Amphibian Oocytes and Embryos. Confocal Fluorescence Microscopy Measurements of pH and Calcium in Living Cells. Confocal and Non-Linear Optical Imaging of Potentiometric Dyes. Measurement and Intracellular Ca2+ Concentration. Running and Setting Up a Confocal Microscope Core Facility.