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Calcium in Living Cells

Edited by
  • Michael Whitaker, Department of Physiological Sciences Medical School, Newcastle upon Tyne, England

Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. This volume will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases.

Audience
Researchers in cell, molecular and developmental biology, biochemists

Included in series
Methods in Cell Biology

Hardbound, 328 Pages

Published: November 2010

Imprint: Academic Press

ISBN: 978-0-12-374841-6

Contents

      1. A Practical Guide to the Preparation of Ca 2+ Buffers

        2. Donald M. Bers, Chris W. Patton and Richard Nuccitelli

      2. Photorelease Techniques for Raising or Lowering Intracellular Ca2+

    Robert Zucker

    3. Making and Using Calcium-Selective Mini- and Microelectrodes

    L. Hove-Madsen, S. Baudet and D.M. Bers

    4. Construction, Theory and Practical Considerations for using Self-Referencing of Ca 2+ -Selective Microelectrodes for Monitoring Extracellular Ca 2+ Gradients

    Mark A. Messerli and Peter J.S. Smith

    5. Practical Aspects of Measuring Intracellular Calcium Signals with Fluorescent Indicators

    Joseph P. Y. Kao, Gong Li, Darryl A. Auston

    6. Genetically-encoded probes for measurement of intracellular calcium

    Michael Whitaker

    7. Patch clamp methods for studying calcium channels

    David L. Armstrong, Christian Erxleben and Jody A. White

    8. Nuclear Patch-Clamp Recording from Inositol 1,4,5-Trisphosphate Receptors

    Taufiq Rahman, Colin W. Taylor

    9. Confocal And Multiphoton Imaging Of Intracellular Ca 2+

    Godfrey Smith, Martyn Reynolds, Francis Burto1, Ole Johan Kemi

    10. The Use of Aequorins to Record and Visualize Ca 2+ Dynamics: From Subcellular Microdomains to Whole Organisms.

    Sarah E. Webb, Kelly L. Rogers, Eric Karplus, and Andrew L. Miller

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