Caenorhabditis Elegans

Molecular Genetics and Development

Edited By

  • Joel Rothman

An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.
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Researchers and students in cell, molecular and developmental biology


Book information

  • Published: November 2011
  • ISBN: 978-0-12-544172-8

Table of Contents

Tentative Table of Contents:

Culturing methods and genetic procedures
General methods of culture: Jim Lewis
Mutagenesis: Phil Anderson
Genetic screening methods: James Thomas, Eric Jorgensen, Eric Lambie
Genetic mapping methods: Ben Williams, Stephen Wicks (Boston College)
Genetic mosaic analysis: Robert Herman
Use of specialized chromosomes and rearrangements: Ann Rose, David Baillie
Gene knockouts: Shohei Mitani, Robert Barstead
RNAi and RNAi screening methods: Julie Ahringer, Andrew Dillin

Genomics and Bioinformatics
Transcriptional profiling: Stuart Kim, Don Moerman, Valerie Reinke
MicroRNA methods: Victor Ambros, Frank Slack, Amy Pasquinelli
Analysis of RNA function: Marv Wickens, Judith Kimble
Analysis of the interactome: Marc Vidal
WormBase: Eric Schwartz, Paul Sternberg, Lincoln Stein
Structural genomics: Ming Luo, Larry DeLucas, Songlin Li, Mike Carson (U. Alabama)
Proteomics: Ahna Skop, Moerman D., Isobe T, Krijgsveld, J
Transcriptional regulatory networks: Marion Walhout

Markers for gene expression and protein localization
Immunofluorescence and antibodies: David Miller
Imaging: John White, William Mohler
Electron Microscopy: David Hall
Proteins and protein assemblies: Henry Epstein
Analysis of morphogenesis: Jeff Hardin, Andrew Chisholm
Cell death: Monica Driscoll, Ding Xue