CAENORHABDITIS ELEGANS: Cell Biology and Physiology

The second part of an updated edition of the classic Methods in Cell Biology, Volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.
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Audience

Researchers and students in cell, molecular and developmental biology

 

Book information

  • Published: January 2012
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-394620-1


Table of Contents

  1. Imaging
  2. 1. High-resolution Imaging of Cellular Processes in C. elegans

    Amy S. Maddox and Paul S. Maddox

    2. Immunofluorescence Microscopy

    Diane C. Shakes, David Miller and Michael L. Nonet

    3. Fluorescent Protein Methods: Strategies and Applications

    Harald Hutter

    4. Modern Electron Microscopy Methods for C. elegans

    David H. Hall, Erika Hartwieg, and Ken C.Q. Nguyen

  3. Cell Biology
  4. 5. Culture and Manipulation of Embryonic Cells

    Lois G. Edgar and Bob Goldstein

    6. Laser Microsurgery in Caenorhabditis elegans

    Christopher Fang-Yen, Christopher V. Gabel, Aravinthan D. T. Samuel, Cornelia I. Bargmann, and Leon Avery

    7. Analysis of Cell polarity

    Tobias Wiesenfahrt, Olaf Bossinger, and Carrie Cowan

    8. Analysis of Organelles

    Hanna Fares and Alex M. van der Bliek

    9. C. elegans Cell Cycle Analysis

    Sander Van den Heuvel and Edward Kipreos

  5. Physiology

10. Methods for Studying Programmed Cell Death in C. elegans

Yi-chun Wu, Xiaochen Wang, and Ding Xue

11. Methods for Studying the DNA Damage Response in the Caenorhabditis elegans Germline

Ashley L. Craig, Sandra C. Moser, Aymeric P. Bailly and Anton Gartner

12. Analysis of Aging in Caenorhabditis elegans

Deepti Srinivas Wilkinson, Rebecca C. Taylor, and Andrew Dillin

13. Analyses of C. elegans Fat Metabolic Pathways

Alexandre Guimarães de Almeida Barros, Jason Liu, George A. Lemiuex, Brendan C. Mullaney, and Kaveh Ashrafi

14. Electrophysiological Methods for C. elegans Neurobiology

Miriam B. Goodman, Theodore H. Lindsay, Shawn R. Lockery, and Janet E. Richmond