Biophotonics, Part B

Edited by

  • Gerard Marriott, University of Wisconsin, U.S.A.
  • Ian Parker, University of California, Irvine

This volume and its companion volume 360 introduce a new topic to the Methods in Enzymology series. They will cover, among other topics, imaging, screening, and diagnosis in biological systems. See key features for greater detail.
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Biochemists, Biophysicists, Physiologists, Cell Biologists, Pharmacologists, Geneticists


Book information

  • Published: February 2003
  • ISBN: 978-0-12-182264-4


"Methods in Enzymology is a highly respected, widely available, and practical series of volumes that contains detailed protocols and descriptions of biochemical and biophysical techniques...They have compiled fifty-three excellent chapters on the broad subject of biophotonics, which were written by internationally renowned scientists who work in twelve countries...The editors of Biophotonics are to be congratulated for having made a judicious selection of both the topics to be covered and also the quality of the authors they have selected to present these topics…I suggest that Biophotonics would be extremely useful to any researcher who is working in this expanding field. Undergraduate students beginning their research projects, graduate students, scientists, engineers, and clinicians involved with biophotonics would comprise the intended audience...I highly recommend Biophotonics as a very reliable and useful reference work...The very high quality illustrations are well documented with legends and annotations...The production quality of these volumes is very high...In summary, the two-volume set of Biophotnics constitutes an extensive set of protocols, techniques, instruments, and methods of analysis that constitute that state-of-the art. The books are extremely well organized, clearly written, profusely illustrated, and thoroughly documented. The editors and the diverse group of authors have succeeded in their aims and have produced a highly useful and recommended set of books." - Journal of Biomedical Optics (2005)

Table of Contents

Total Internal Reflection Fluorescence Microscopy in Cell BiologyReflection Interference Contrast MicroscopySecond Harmonic Imaging MicroscopyFluorescence Correlation SpectroscopyFluorescence Correlation Spectroscopy of GFP Fusion Proteins in Living Plant CellsBuilding and Using Optical Traps to Study Properties of Molecular MotorsOptical-Trap Force Transducer That Operates by Direct Measurement of Light MomentumApplication of Optical Traps in VivoCytomechanics Applications of Optical Sectioning MicroscopyMeasurements of Cell-Generated Deformations on Flexible Substrata Using Correlation-Based Optical FlowSingle-Molecule Imaging of Rotation of F1-ATPaseMolecular Motors and Single-MoleculeVisualization of Single Molecules of mRNA in SituSingle Ion Channel ImagingGFP-Fusion Proteins as Fluorescent Reporters to Study Organelle and Cytoskeleton Dynamics in Chemotaxis and PhagocytosisDynamic Imaging of Cell-Substrate ContactsImaging Mitochondrial Function in Intact CellsDynamic Imaging of Neuronal CytoskeletonImaging Calcium Dynamics in Developing NeuronsOptical Monitoring of Neural Activity Using Voltage-Sensitive DyesSteady-State Fluorescence Imaging of NeoplasiaIn Vivo and ex Vivo Tissue Applications of Two-Photon MicroscopyFluorescence Anisotropy in Pharmacologic ScreeningSurface and Printing Effects on Fluorescent Images of Immobilized Biomolecule Arrays