Table of ContentsGastrointestinal System:Salivary Gland:I. Novak, Salivary Secretion: Studies on Intact Glands in Vivo and in Vitro.K. Haag and H. Knauf, Determination of Short-Circuit Current in Small Tubular Structures via Cable Analysis.B.J. Baum, I.S. Ambudkar, J. Helman, V.J. Horn, J.E. Melvin, L.M. Mertz, and R.J. Turner, Dispersed Salivary Gland Acinar Cell Preparations for use in Studies of Neurorecptor-Couples Secretory Events.Stomach:P.A. Negulescu and T.E. Machen, Intracellular Ion Activities and MembraneTransport in Parietal Cells Measured with Fluorescent Probes.S. Curci and E. Fromter, Electrophysiological Techniques in Analysis of ion Transport across Gastric Mucosa.T. Berglindh, Gastric Glands and Cells: Preparation and in Vitro Methods.D.H. Malinowska, Permeabilizing Parietal Cells.S.J. Hersey, L. Tang, J. Pohl, and M. Miller, Pepsinogen Secretion in Vitro.G. Flemstrom and H. Forssell, HCO3 Secretion: Stomach and Duodenum.W.W. Reenstra and J.G. Forte, Isolation of H+,K+-ATPase-Containing Membranes from the Gastric Oxyntic Cell.B.E. Peerce, Peptic Granules from Stomach.T. Yamada, Isolation and Primary Culture of Endocrine Cells from Canine Gastric Mucosa.Pancreas:C.B. Wollheim, P. Meda, and P.A. Halban, Isolation of Pancreatic Islets and Primary Culture of Intact Microorgans or of Dispersed Islet Cells.C.B. Wollheim, P. Meda, and P.A. Halban, Establishment and Culture of Insulin-Secreting B-Cell Lines.H.P. Meissner, Membrane Potential Measurements in Pancreatic B-Cells with intracellular Microelectrodes.S.A. Wank, R.T. Jensen, and J.D. Gardner, Stimulation of Secretion by Secretagogues.R.M. Case and B.E. Argent, In Vivo, Perfused Gland, and Isolated Duct Studies of Pancreatic Secretion.D. Menozzi, R.T. Jensen, and J.D. Gardner, Dispersed Pancreatic Acinar Cells and Pancreatic Acini.I. Schulz, Permeabilizing Cells and Subcellular Fractionation: Role of Inositol Trisphosphate, Diacylglycerol,and Calcium as Intracellular Messengers in Stimulus- Secretion Coupling.O.H. Peterson, Electrophysiology of Pancreas and Exodrine Tear Duct.Intestine:M. Duffey, Transport Mechanisms and Control of Secretion.G.A. Kimmich, Isolation of Intestinal Epithelial Cells and Evaluation of Transport Functions.A.K. Mircheff and E.J.J.M. van Corven, Isolation of Enterocyte Membranes.K. Dharmsathaphorn and J.L. Madara, Established Intestinal Cell Lines as Model Systems for Electrolyte Transport Studies.U. Hopfer, Sodium Chloride Pathways in Gut Vesicles.A. Berteloot and G. Semenza, Advantages and Limitations of Vesicles for Characterization and Kinetic Analysis of Transport Systems.E.M. Wright, Glucose Carrier Cloning by Expression.P. Malathi and M. Takahashi, Isolation and Reconstitution of Sodium-Dependent Glucose Transporter.J.R.F. Walters and M.M. Weiser, Calcium Transport by Intestinal Epithelial Cell Basolateral Membranes.U. Hegel, Gastrointestinal System: Large intestine (including Colon, Rectum, Cecum).M. Ookhtens and N. Kaplowitz, Use of Isolated Perfused Liver and Studies of Biological Transport Processes.L. Combettes, C. Dargemont, J.-P. Mauger, and M. Claret, Measurement of Unidirectional Calcium Fluxes in Liver.J.L. Boyer, J.M. Phillips, and J. Graf, Preparation and Specific Applications of isolated Hepatocyte Couplets.J.L. Boyer and P.J. Meier, Characterizing Mechanisms of Hepatic Bile Acid Transport Utilizing Isolated Membrane Vesicles.P.J. Meier and J.L. Boyer, Preparation of Basolateral (Sinusoidal) and Canalicular Plasma Membrane Vesicles for Study of Hepatic Transport Processes.Other Epithelia:Trachea:C.M. Liedtke, Electrogenic and Electroneutral Ion Transporters and Their Regulation in Tracheal Epithelium.J.R. Yankaskas and R.C. Boucher, Transformation of Airway Epithelial Cells with Persistence of Cystic Fibrosis or Normal Ion Transport Phenotypes.D. Landry, Purification and Reconstitution of Chloride Channels from Kidney and Trachea.Cornea and Eye:J.A. Zadunaisky, Transport in Epithelium and Endothelium.M. Wiederholt and T.J. Jentsch, Cell Culture of Bovine Corneal Endothelial Cells and Its Application to Transport Studies.Sweat Glands:K. Sato and F. Sato, Eccrine Sweat Gland Function in Vivo and in Vitro.Insect Epithelia:W.R. Harvey, D.N. Crawford, and D.D. Spaeth, Isolation, Voltage Clamping, and Flux Measurements in Lepidopteran Midgut.H. Wieczorek, M. Cioffi, U. Klein, W.R. Harvey, H. Schweikl, and M.G. Wolfersberger, Isolation of Goblet Cell Apical Membrane from Tobacco Hormwork Midgut and Purification of its Vacuolar-Type ATPase.S.H.P. Maddrell and J.A. Overton, Fluid and Solute Transport and Their Control in Insect Malpighian Tubules.Urinary Bladder:S.A. Lewis and J.W. Hanrahan, Physiological Approaches for Studying Mammalian Urinary Bladder EpitheliumGallbladder:G. Altenberg, J. Copello. C. Cotton, K. Dawson, Y. Segal, F. Wehner, and L. Reuss, Electrophysiological Methods for Ion and Water Transport in Necturus Gallbladder Epithelium.Amphibian and Reptilian Epithelia:M.M. Civan and H. Garty, Toad Urinary Bladder as a Model for Studying Transepithelial Sodium Transport.L.G.M. Gordon, G. Kottra, and E. Fromter, Detection, Quantification and Minimization of Edge Leaks in Using Chambers.H. Oberleithner, B. Gabner, P. Dietl, and W. Wang,Amphibian Nephron: Isolated Kidney and Cell Fusion.D.C. Dawson and D. Chang, Turtle Colon: Keeping Track of Transporters in Apical and Basolateral Membranes.Fish Epithelia:M.W. Musch, S.M. O'Grady, and M. Field, Ion Transport of Marine Teleost Intesting.P. Silva, R.J. Solomon, and F.H. Epstein, Shark Rectal Gland.J.A. Zadunaisky, Gills: Chloride Secretory Cells.