Biomedical Optical Phase Microscopy and Nanoscopy book cover

Biomedical Optical Phase Microscopy and Nanoscopy

Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy

The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications.


Biomedical engineers and researchers
Nanotechnology engineers and researchers
Optical engineers and researchers

Microscopy engineers

Hardbound, 432 Pages

Published: November 2012

Imprint: Academic Press

ISBN: 978-0-12-415871-9


  • "This interesting and inspiring book is a collection of articles on specific applications in optical imaging of cells and tissues…Phase imaging is understood in a broad sense and related techniques of super-resolution, polarization and optical coherence microscopy are described in a concise but very informative manner."--Optics & Photonics News Online, June 13, 2013

    "This book has been structured to be self-contained and therefore useful for researchers and students at all levels who need a cutting edge review of techniques and applications, particularly biological and medical applications."--Reference and Research Book News, December 2012


  • Preface
    Natan T. Shaked, Zeev Zalevskey and Lisa L. Satterwhite

    1. Phase contrast microscopy and differential interference contrast microscopy
    2. Chapter 1

      Principles of phase contrast microscopy

      Sam A. Johnson

      Editor: Lisa L. Satterwhite

      Chapter 2

      Differential interference contrast (DIC) microscopy

      Michael Shribak
      Editor: Lisa L. Satterwhite

      Chapter 3

      Long term phase contrast imaging of untransformed human cells

      Yumi Uetake and Greenfield Sluder

      Editor: Lisa L. Satterwhite

      Chapter 4

      Phase imaging of plant cells and tissues

      Vassilios Sarfis
      Editor: Zeev Zalevsky

    3. Digital holographic phase microscopy
    4. Chapter 5

      Measuring biophysical parameters of living cells with digital holographic microscopy

      Benjamin Rappaz, Christian Depeursinge and Pierre Marquet

      Editor: Natan T. Shaked

      Chapter 6

      New digital holographic microscopy setups and techniques for obtaining multi-focus quantitative phase imaging of living cells

      Björn Kemper, Patrik Langehanenberg and Andreas Bauwens

      Editor: Natan T. Shaked

      Chapter 7

      Phase unwrapping problems in digital holographic microscopy of biological cells
      Alexander Khmaladze

      Editor: Natan T. Shaked

      Chapter 8

      Partially-coherent lensless holographic microscopy on a chip and its application for automated semen analysis

      Ting-Wei Su and Aydogan Ozcan

      Editor: Natan T. Shaked

      Chapter 9

      Synthetic aperture lensless digital holographic microscopy for superresolved biological imaging

      Vicente Micó, Zeev Zalevsky, Luis Granero and Javier García

      Editor: Zeev Zalevsky

      Chapter 10

      Combining digital holographic microscopy with microfluidics: a new tool in biotechnology.

      Lisa Miccio, Pasquale Memmolo, Fraancesco Merola, Melania Paturzo and Pietro Ferraro

      Editor: Zeev Zalevsky

      Chapter 11

      Holographic motility contrast imaging of live tissues

      David D. Nolte, Ran An, Kwan Jeong and John Turek

      Editor: Natan T. Shaked


    5. Advanced interferometric and polarization techniques
    6. Chapter 12

      Tomographic phase microscopy

      Wonshik Choi

      Editor: Natan T. Shaked

      Chapter 13

      Phase-sensitive optical coherence tomography

      Itay Shock and Natan T. Shaked

      Editor: Natan T. Shaked

      Chapter 14

      Polarization and spectral interferometric techniques for quantitative phase microscopy

      Yizheng Zhu, Matthew T. Rinehart, Francisco E. Robles and Adam Wax

      Editor: Natan T. Shaked


      Chapter 15

      Polarization microscopy
      Rudolf Oldenbourg
      Editor: Lisa L. Satterwhite


    7. Phase nanoscopy

    Chapter 16

    Is there a fundamental limit to optical resolution in phase microscopy?
    Steve Lipson

    Editor: Zeev Zalevsky

    Chapter 17

    Nano-holographic interferometry for in-vivo observations
    Federico Sciammarella, Cesar A. Sciammarella and Luciano Lamberti

    Editor: Natan T. Shaked

    Chapter 18

    Fluorescence phase microscopy
    Alberto Bilenca, Brett Bouma, Guillermo Tearney, Iwan Märki, Noelia Bocchio,

    Stefan Geissbuehler and Theo Lasser

    Editor: Natan T. Shaked


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