The Bacteria book cover

The Bacteria

Molecular Basis of Bacterial Pathogenesis

This volume of Methods in Enzymology contains contributions covering the wide spectrum of interactions between bacterial pathogens and their eukaryotic hosts. It complements Volume 235, in which methods for the isolation and identification of bacterial pathogens and associated virulence determinants are described. Since the study of bacterial pathogenesis borrows from a great variety of technologies and disciplines, we aim to provide the reader with a representative sample of the most advanced pathogenesis research.

Audience
Microbiologists, molecular biologists, biochemists, geneticists, pathologists, and immunologists (from MIE 235).

Included in series
Methods in Enzymology

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Published: July 1994

Imprint: Academic Press

ISBN: 978-0-12-182137-1

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Contents

  • Bacterial Effects on Immune Cells:H. Loppnow, H. Brade, E.T. Rietschel, and H.-D. Flad, Induction of Cytokines in Mononuclear and Vascular Cells by Endotoxin and Other Bacterial Products.M.S. Detrick, R. Kreisberg, and R.N. Moore, Effect of Bacterial Products on Colony-Stimulating Factor Production.Z.A. McGee and C.M. Clemens, Effect of Bacterial Products on Tumor Necrosis Factor Production: Quantitation in Biologic Fluids or Tissues.K.-I. Tanamoto, Induction of Prostaglandin Release from Macrophages by Bacterial Endotoxin.G. Sonnenfeld, Effect of Bacterial Products on Interferon Production.J.E. Puzas, D.G. Hicks, S.D. Reynolds, and R.J. OKeefe, Regulation of Osteoclastic Activityin Infection.J.R. Kalmar and T.E. Van Dyke, Effect of Bacterial Products on Neutrophil Chemotaxis.Phagocytosis and Killing Assays:R.F. Rest and D.P. Speert, Measurement of Nonopsonic Phagocytic Killing by Human and Mouse Phagocytes.J.R. Kalmar, Measurement of Opsonic Phagocytosis by Human Polymorphonuclear Neutrophils.R.F. Rest, Measurement of Human Neutrophil Respiratory Burst during Phagocytosis of Bacteria. D.P. McQuillen, S. Gulati, and P.A. Rice, Complement-Mediated Bacterial Killing Assays.T.H. Steinberg and J.A. Swanson, Measurement of Phagosome-Lyosome Fusion and Phagosomal pH.S.S.L. Harwig, T. Ganz, and R.I. Lehrer, Neutrophil Defensins: Purification, Characterization, and Antimicrobial Testing.J. Weiss, Purification and Assay of Bactericidal/Permeability-Increasing Protein.G. Storz and M.B. Toledano, Regulation of Bacterial Gene Expression in Response to Oxidative Stress.Adherence:J. Goldhar, Bacterial Lectinlike Adhesins: Determination and Specificity.R.E. Mandrell, M.A. Apicella, R. Lindstedt, and H. Leffler, Possible Interaction between Animal Lectins and Bacterial Carbohydrates.S.A. Kinder and S.C. Holt, Coaggregation between Bacterial Species.G.K. Schoolnik, Purification of Somatic Pili.M.J. Kuehn, F. Jacob-Dubuisson, K. Dodson, L. Slonim, R. Striker, and S.J. Hultgren, Genetic, Biochemical, and Structural Studies of Biogenesis of Adhesive Pili in Bacteria.G.T. Van Nhieu and R.R. Isberg, Isolation and Identification of Eukaryotic Receptors Promoting Bacterial Internalization.J.F. Alderete, R. Arroyo, and M.W. Lehker, Identification of Fibronectin as a Receptor for Bacterial Cytoadherence.E. Rozdzinski and E. Tuomanen, Interactions of Bacteria with Leukocyte Integrins.C.D. Deal and H.C. Krivan, Solid-Phase Binding of Microorganisms to Glycolipids and Phospholipids.P. Falk, T. Boren, and S. Normark, Characterization of Microbial Host Receptors.Invasion and Intracellular Survival in Eukaryotic Cells:J. Schachter, Culture and Isolation of Chlamydia trachomatis.D.L. Cox, Culture of Treponema pallidum.E.A. Elsinghorst, Measurement of Invasion by Gentamicin Resistance.J.P.M. Van Putten, J.F.L. Weel, and H.U.C. Grassme, Measurements of Invasion by Antibody Labeling and Electron Microscopy.M.G. Pucciarelli and B.B. Finlay, Polarized Epithelial Monolayers: Model Systems to Study Bacterial Interactions with Host Epithelial Cells. D.A. Haake and M.A. Lovett, Interjunctional Invasion of Endothelial Cell Monolayers.S. Jones and D.A. Portnoy, Intracellular Growth of Bacteria.I. Rosenshine, S. Ruschkowski, and B.B. Finlay, Inhibitors of Cytoskeletal Function and Signal Transduction to Study Bacterial Invasion.L.G. Tilney and M.S. Tilney, Methods to Visualize Actin Polymerization Associated with Bacterial Invasion.J.L. Pace and J.E. Galan, Measurement of Free Intracellular Calcium Levels in Epithelial Cells as a Consequence of Bacterial Invasion.Identification of Genes Involved in Invasion:R. Menard and P.J. Sansonetti, Shigella flexneri: Isolation of Noninvasive Mutants of Gram-Negative Pathogens.F. Bowe and F. Heffron, Isolation of Salmonella Mutants Defective for Intracellular Survival.S. Jones and D.A. Portnoy, Small Plaque Mutants.C.A. Lee and S. Falkow, Isolation of Hyperinvasive Mutants of Salmonella.V.L. Miller and B.J. Stone, Molecular Cloning of Invasion Genes from Yersinia and Salmonella.J.-L. Gaillard, S. Dramsi, P. Berche, and P. Cossart, Molecular Cloning and Expression of Internalin in Listeria.S. Rankin, G.T. Van Nhieu, and R.R. Isberg, Use of Staphylococcus aureus Coated with Invasin Derivatives to Assay Invasin Function.Author Index.Subject Index.

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