Aqueous Size-Exclusion Chromatography
- P.L. Dubin, Indiana-Purdue University, Indianapolis, USA
The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses.These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole.This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating.View full description
- Published: May 1988
- Imprint: ELSEVIER
- ISBN: 978-0-444-42957-5
Table of ContentsPart I. Separation Mechanisms. Size exclusion parameters (M.E. Himmel, P.G. Squire). Partitioning: Hydrophobic interactions (M. Janado). Electrostatic effects (P.L. Dubin). Exclusion chromatography of inorganic compounds (M. Shibukawa, N. Ohta). II. Characterization of Stationary Phases. Pore size distributions (L. Hagel). Structural analysis of porous materials by measurement of size exclusion (S. Kuga). Column efficiency (S. Mori). III. New Packings. Native and bonded silicas in aqueous SEC (K.K. Unger, J.N. Kinkel). Rigid polymer gels for SEC and their application to biopolymers (K. Makino, H. Hatano). IV. Biopolymers. Biopolymers. I. Protein chromatography in denaturing and non-denaturing solvents (R.C. Montelaro). Biopolymers. II. Serum lipoproteins (M. Okazaki, I. Hara). Application of SEC/LALLS to biopolymer assemblies (K. Konishi). V. Associating Systems. Measurement of equilibrium constants by exclusion methods (T.K. Korpela, J.-P. Himanen). 14. Frontal boundary analysis in size exclusion chromatography of self-associating proteins (G.W. Becker). 15. Exclusion chromatography of micelles (K.S. Birdi). Subject Index.