Antibody Techniques

Edited By

  • Vedpal Malik, U.S. Department of Agriculture, APHIS, Hyattsville, Maryland, U.S.A.
  • Erik Lillehoj, The George Washington University Medical Center, Washington, D.C., U.S.A.

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Audience

Researchers and technicians in molecular biology, cell biology, developmental biology, virology, microbiology, pharmacology, and biotechnology.

 

Book information

  • Published: September 1994
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-466460-9


Table of Contents

I.M. Zitron, Antibody Molecules and the Immune Response.D.P. Snider, Adjuvant-Free Polyclonal Antibody Response Manipulated by Antibody-Mediated Antigen Targeting.C. Barrett, Hybridomas and Monoclonal Antibodies.J.W. Larrick and R.F. Balint, PCR Amplification and Antibody Genes.P.C. Allen, Laboratory Animal Care and Use.A.C. Allison and N.E. Byars, Adjuvants.C. Glad and I. Nilsson, Large-Scale Production of Monoclonal Antibodies.D.P. Boyle and D.W. Metzger, Antibody-Binding Bacterial Proteins as Immunoreagents.B.-K. Choe, Conjugating Antibodies to Ligands, Enzymes, and Metallic Particles.E. Lauritzen, H. Flyge, and A. Holm, Dot Immunobinding (DIB), Enzyme Linked Immunosorbent Assay (ELISA) and Radioimmunoassay (RIA) for Detection of Peptide Antigens and Specific Antibodies.B.-K. Choe and Y. Cho, Immunostaining Cells and Tissues.E.P. Lillehoj, Protein Immunoblotting.H.S. Lillehoj and A.G. Martin, Flow Cytometry and Fluoresence-Activated Cell Sorting.W.J. Patrie, Screening Expression Libraries with Antibodies.J.P. Tharakan, Immunoaffinity Purification.