Algal Culturing Techniques

Edited by

  • Robert Andersen, Provasoli-Guillard National Center for Culture of Marine Phytoplankton, West Boothbay Harbor, ME USA

Algal Culturing Techniques is a comprehensive reference on all aspects of the isolation and cultivation of marine and freshwater algae, including seaweeds. It is divided into seven parts that cover history, media preparation, isolation and purification techniques, mass culturing techniques, cell counting and growth measurement techniques, and reviews on topics and applications of algal culture techniques for environmental investigations.Algal Culturing Techniques was developed to serve as both a new textbook and key reference for phycologists and others studying aquatic systems, aquaculture and environmental sciences. Students of algal ecology, marine botany, marine phycology, and microbial ecology will enjoy the hands-on methodology for culturing a variety of algae from fresh and marine waters. Researchers in industry, such as aquaculture, pharmaceutical, foodstuffs, and biotechnology companies will find an authoritative and comprehensive reference.
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Scientists, industry professionals, and students who study or grow algal cultures for research or commercial purposes. Libraries, aquaculturists, biotechnologists, governmental scientists, etc.


Book information

  • Published: January 2005
  • ISBN: 978-0-12-088426-1


"It has been worth the wait. Andersen and the authors have done a tremendous job bringing together many old and new methods in highly readable and easy to follow order...In addition to the older standard methods, it adds many new ones, from automated isolation and counting of algal cells to use of cultures as a way to prevent extinctions of threatened species...the methods chapters, e.g., Andersen and Kawachi on microalgal isolation techniques are very detailed, providing plain, easy to follow, often step-by-step instructions, complete with supplies needed and even sources, on how to do particular tasks...Bob Andersen, the authors, and PSA are to be commended for bringing together so much detailed information in one place. The book is beautifully produced...It definitely needs to be in the lab and/or office of everyone doing research on algae...I highly recommend it." - R.A. Horner, University of Washington, School of Oceanography, Seattle, U.S.A., in HARMFUL ALGAE (2006) "This comprehensive book is a necessity for research scientists who use and maintain algal cultures." CHOICE (October 2005)

Table of Contents

ContributorsPreface1. Historical Review of Algal Culturing Techniques2. Freshwater Culture Media3. Marine Culture Media4. Trace Metal Ion Buffers and Their Use in Culture Studies5. Sterilization and Sterile Technique6. Traditional Microalgae Isolation Techniques7. Automated Isolation Techniques for Microalgae8. Purification Methods for Microalgae9. Isolation and Purification Techniques for Macroalgae10. Perpetual Maintenance of Actively Metabolizing Microalgal Cultures11. Long Term Macroalgal Culture Maintenance12. Cryopreservation Methods for Maintaining Microalgal Cultures13. Photobioreactors and Fermentors: The Light and Dark Sides of Growing Algae14. Culturing Microalgae in Outdoor Ponds15. Mariculture of Seaweeds16. Counting Cells in Culture Using the Light Microscope17. Phytoplankton Cell Counting by Flow Cytometry18. Measuring Growth Rates in Microalgal Cultures19. Using Cultures to Investigate the Physiological Ecology of Microalgae20. Analysis of Algal Pigments by High-Performance Liquid Chromatography21. Endogenous Rhythms and Daylength Effects in Macroalgal Development22. Viral Contamination of Algal Cultures23. Control of Sexual Reproduction in Algae in Culture24. Microalgal Life Cycle Dynamics: Encystment and Excystment25. Cultures as a Means of Protecting Biological Resources: Ex-situ Conservation of Threatened Algal SpeciesAppendix A: Recipies for Freshwater and Seawater MediaGlossaryName IndexSubject Index