A Practical Guide to Protein and Peptide Purification for Microsequencing

Edited by

  • Paul Matsudaira, Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
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Researchers and students at all levels in cell, developmental, and molecular biology, protein biochemistry and biophysics, neurobiology, pharmacology, immunology, parasitology, virology, genetics, plant science, biotechnology, and other biomedical/biological areas.


Book information

  • Published: May 1993
  • ISBN: 978-0-12-480282-7


"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world."
From the Reviews of the First Edition, --CELL

Table of Contents

Introduction. Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation. Purification of Proteins and Peptides By SDS-PAGE. Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose. Mass Spectrometric Strategies for the Structural Characterization of Proteins. Appendix. References. General Reference Books on Protein Microsequencing Methodologies.